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For our 16S work, only one amplicon per individual was present in each pool and its length of 420-bp was well within the GS FLX instruments modal read length (477-bp). BMC Genomics 11: 296. BEHEMOTH PROVOKE INTENSE REACTION WITH NEW IMAGE "Everyone on here talking about no longer being hungry, I am over here getting more hungry." . DiscussionWe have evaluated the accuracy and feasibility of a new approach for population genetics in an ecological and conservation context parallel tagged amplicon NGS on pooled population samples. We explored further possible outcomes of our NGS pooled population samples data for both 16S and Cyt b using phylogenetic and molecular evolutionary analyses with MEGA version 4 . Not surprisingly, the positive association (R2) decreased, however only slightly (Table S2), indicating that low within-population variation had minimal effect on the differences observed between two mitochondrial genes.  and based on the mean number of differences between different sequences sampled from the same population and sampled from two different populations. Related Song. Grueber, Neil J. N=20 population estimates), as well as the estimates of pairwise population differentiation (FST) under the two approaches (i.e.